non targeting nt control sgrna (Addgene inc)
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Addgene inc
non targeting nt control sgrna
Non Targeting Nt Control Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/non+targeting+sgrna/lentiCRISPR+v2+(Plasmid+%2352961)/pm41840739-171-12-20
Average 96 stars, based on 6181 article reviews
Non Targeting Nt Control Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/non+targeting+sgrna/lentiCRISPR+v2+(Plasmid+%2352961)/pm41840739-171-12-20
Average 96 stars, based on 6181 article reviews
non targeting nt control sgrna - by Bioz Stars,
2026-10
96/100 stars
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Plasmid Preparation:Article Title: Lysine-specific demethylase 1 regulates hematopoietic stem cell expansion and myeloid cell differentiation Article Snippet: For CRISPR-Cas9 gene editing, THP1 and K562 cells were initially transduced with pLenti-Cas9-P2A-Puro (Addgene #110837), and cells gaining stable Cas9 expression were selected by culture in 2 μg/ml Puromycine. .. In a second step, LSD1 sgRNAs or a Article Title: CRISPR/Cas9-Mediated Targeting of BPV-1-Transformed Primary Equine Sarcoid Fibroblasts. Article Snippet: Clones with the correct insert were amplified and plasmids for lentivirus production were purified using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research, USA). .. Lentivirus production was performed using calcium-phosphate-mediated transfection of HEK-293 T-cells (15 cm dish, Corning, Corning, NY, USA) with the plentiCRISPRv2 vector containing the respective sgRNA or a Article Title: miR-10b-5p regulates adipocyte lineage commitment and adipogenesis via targeting of Gata6 and Tubby Article Snippet: The chosen single-stranded oligonucleotides containing the guide sequence of the gRNA were annealed and cloned into the BbsI linearised px330-U6-Chimeric_BB_CBh-huSpCas9 vector (Addgene plasmid # 42230); ( ). .. The Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer Article Snippet: Human SCLC cell line H1048 was obtained from ATCC and cultured in RPMI media supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, and 1% penicillin/streptomycin antibiotic cocktail. .. To generate H1048 sg NTC, sg PTEN, and myrAKT cell lines, cells were infected with a Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer Article Snippet: .. To generate Pten- knockout RPM and RPMA organoids, basal organoids transformed by CMV-Cre were infected with a Clone Assay:Article Title: Biphasic control of the B cell transcriptome by mTORC1 and GSK3 Article Snippet: .. For this, selected sgRNAs and a Article Title: Patient derived model of UBA5- associated encephalopathy identifies defects in neurodevelopment and highlights potential therapies Article Snippet: .. Additionally, a Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer Article Snippet: .. To generate Pten- knockout RPM and RPMA organoids, basal organoids transformed by CMV-Cre were infected with a other:Article Title: Biphasic control of the B cell transcriptome by mTORC1 and GSK3. Article Snippet: Shalem, O., Sanjana, N.E., Hartenian, E., Shi, X., Scott, D.A., Mikkelson, T., Heckl, D., Ebert, B.L., Root, D.E., Doench, J.G., and Zhang, F. (2014). Transfection:Article Title: CRISPR/Cas9-Mediated Targeting of BPV-1-Transformed Primary Equine Sarcoid Fibroblasts. Article Snippet: Clones with the correct insert were amplified and plasmids for lentivirus production were purified using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research, USA). .. Lentivirus production was performed using calcium-phosphate-mediated transfection of HEK-293 T-cells (15 cm dish, Corning, Corning, NY, USA) with the plentiCRISPRv2 vector containing the respective sgRNA or a Negative Control:Article Title: miR-10b-5p regulates adipocyte lineage commitment and adipogenesis via targeting of Gata6 and Tubby Article Snippet: The chosen single-stranded oligonucleotides containing the guide sequence of the gRNA were annealed and cloned into the BbsI linearised px330-U6-Chimeric_BB_CBh-huSpCas9 vector (Addgene plasmid # 42230); ( ). .. The Article Title: Patient derived model of UBA5- associated encephalopathy identifies defects in neurodevelopment and highlights potential therapies Article Snippet: .. Additionally, a Infection:Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer Article Snippet: Human SCLC cell line H1048 was obtained from ATCC and cultured in RPMI media supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, and 1% penicillin/streptomycin antibiotic cocktail. .. To generate H1048 sg NTC, sg PTEN, and myrAKT cell lines, cells were infected with a Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer Article Snippet: .. To generate Pten- knockout RPM and RPMA organoids, basal organoids transformed by CMV-Cre were infected with a Construct:Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer Article Snippet: Human SCLC cell line H1048 was obtained from ATCC and cultured in RPMI media supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, and 1% penicillin/streptomycin antibiotic cocktail. .. To generate H1048 sg NTC, sg PTEN, and myrAKT cell lines, cells were infected with a Knock-Out:Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer Article Snippet: .. To generate Pten- knockout RPM and RPMA organoids, basal organoids transformed by CMV-Cre were infected with a Transformation Assay:Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer Article Snippet: .. To generate Pten- knockout RPM and RPMA organoids, basal organoids transformed by CMV-Cre were infected with a |
